A recent article (https://dash.harvard.edu/handle/1/42639016) has suggested the intriguing and ethically charged possibility of testing the efficacy of candidate SARS-CoV2 vaccines on healthy volunteers. The authors suggest testing candidate vaccines for efficacy by dosing the vaccines to healthy volunteers, infecting those…
Plate 8000 cells/well (2.5*10^4/cm2) of each cell line in 70 uL/well media in a 96 well format (2 x 48 wells per cell line—4 replicates each x 12 doses x 2 drugs). Make a mastermix for 48*1.1 wells and aliquot…
We set up a titration testing 11 concentrations each of remdesivir and hydroxychloroquine (HCQ) each on 4 cell lines (HepG2 and PLC/PRF/5 liver lines, HCT116 and HT29 intestinal lines). Our primary goal is to find a CC50 concentration, defined by…
A frantic week kicked off on Sunday, March 22 when we had our first Google Hangouts group call pitching and brainstorming ideas. In the week that has followed, our lab has accomplished a dizzying array of tasks of all kinds…
Like so many people around the world, the 12 members of our lab have been following the global spread of the SARS-CoV2 coronavirus pandemic and wondering whether we can help out in any way. None of us are doctors. The…