While analysis of our CRISPR screens and initial RdRP experiments are still ongoing, we are making progress analyzing and following up on the RNA-seq data on remdesivir and HCQ host response. HCQ: RNA-seq had identified upregulation of cholesterol metabolism genes…
This week, we have continued analyzing our RNA-seq data, we have completed and sequenced our CRISPR screens, and we have cloned and are performing initial RdRP experiments. Stay tuned for updates on the CRISPR screen in the next few days.…
Here’s an update on our progress this week: RNA-seq We are actively analyzing and following up on the RNA-seq data we collected last week. We have uploaded the raw data and some processed files run through gene (DESeq2) and pathway…
**Note: We are currently searching for SARS-CoV2 genomic RNA or cDNA for use in our project! Our order from ATCC has been backordered until October and we are looking for an alternative. Please reach out to our lab if you…
Fewer than two weeks after starting our experiments aimed at assessing genetic pathways mediating host toxicity of the SARS-CoV2 drugs remdesivir and hydroxychloroquine (HCQ), we already have our first experiment finished. Here’s an update on our progress this week: RNA-seq…
We finished our first titration of remdesivir and HCQ, and, as is so typical, the conclusion was that we need to do it again. That’s not the full story though. We did determine optimal doses for our RNA-seq experiment, for…